KINETIC STUDIES ON ALKALINE PHOSPHATASE FROM ECHINOPLUTEI1

Escherichia coli alkaline phosphatase. Kinetic studies with the tetrameric enzyme.

1. The stability of the tetrameric form of Escherichia coli alkaline phosphatase was examined by analytical ultracentrifugation. 2. The stopped-flow technique was used to study the hydrolysis of nitrophenyl phosphates by the alkaline phosphatase tetramer at pH7.5 and 8.3. In both cases transient product formation was observed before the steady state was attained. Both transients consisted of th...

Kinetic Studies of Alkaline Phosphatase from the Liver of Agama agama Lizard

Kinetic studies were carried out on alkaline phosphatase (ALP) extracted from the liver of Agama agama lizard. Incubation of ALP extract with para – nitrophenyl phosphate formed the basis for the determination of enzyme activity. Spectrophotometric method was used to assay the enzyme, and the kinetic constants: Maximum velocity (Vmax) and Michaelis Menten constant (Km) were calculated. The Km a...

On the mechanism of inhibition of intestinal alkaline phosphatase by L-phenylalanine. I. Kinetic studies.

The degree of inhibition of rat intestinal alkaline phosphatase by L-phenylalanine was highly pa-dependent and varied from 0 to 66% within a pH range of ‘7.8 to 10.4, exhibiting a peak at pH 9.2 and 8.7 for phenylphosphate and /3glycerophosphate, respectively. Vm,, was also a function of pH with and without the inhibitor. Rat intestinal alkaline phosphatase exhibited maximum enzyme activity at ...

Influence of Subunit Interface Mutations on Kinetic and Dynamic Properties of Alkaline Phosphatase from E. coli

Mutations, replacing amino acids involved in the formation of hydrogen bonds between subunits of dimeric alkaline phosphatase, have been introduced. Influence of mutations on kinetic properties and structural stability of mutant enzymes was established. In addition, alterations in protein dynamic properties have been studied using room temperature phosphorescence. Kinetic properties of both mut...

Zone electrophoretic studies of serum alkaline phosphatase.